a KEGG pathway enrichment analysis was performed in Huh-7 cells treated with EM-2 (4 μM). b Huh-7 cells were exposed to EM-2 (0, 2, 4, and 8 μM) for 24 h, and the expression levels of proteins associated with the MAPK pathway were analyzed by Western blotting. c Huh-7 cells were exposed to 4 μM EM-2 for 0 to 36 h, and the protein levels of ERK and p-ERK were analyzed by Western blotting. Representative immunoblot of an experiment repeated three times is shown.