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. 2020 Dec 14;42(7):1139–1149. doi: 10.1038/s41401-020-00564-6

Fig. 5. EM-2 induced endoplasmic reticulum stress in Huh-7 hepatoma cells.

Fig. 5

a Huh-7 cells were exposed to 0, 2, 4, or 8 μM EM-2 for 24 h. The protein expression levels of proteins associated with endoplasmic reticulum stress were detected by Western blotting. b Huh-7 cells were exposed to 0, 2, 4, or 8 μM EM-2 for 24 h. The mRNA expression levels of ER stress-related pathway genes were analyzed by RT-qPCR. All experiments were performed in three independent replicates (*P < 0.05; **P < 0.01; ***P < 0.001).