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. 2021 Jun 16;12:3675. doi: 10.1038/s41467-021-23961-9

Fig. 5. Effect of CTxB on co-clustering of GM1 species with the GPI-anchored protein CD59.

Fig. 5

a Single molecule localization microscopy of mCherry-CD59 and Alexa488-GM1 C16:0 in the presence or absence of CTxB for PS-sufficient cells. b Cross-correlation functions, C(r), of mCherry-CD59 and Alexa488-GM1 C16:0 in PS-sufficient (left) or PS-deficient (right) cells, with (open symbols) or without CTxB (solid symbols). c mCherry-CD59 and Alexa488-GM1 C16:1 do not co-cluster upon CTxB binding. d Cross-correlation functions, C(r), of mCherry-CD59 and Alexa488-GM1 C16:1 (black squares) and C16:0 (red circles) in the presence of CTxB. Scale bars in a and c: top 1.5 μm, bottom 500 nm. Error bars in b and d represent SEM between the cells. n = 9 cells from 3 independent experiments for b and d.