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. 2021 Jun 16;11:12623. doi: 10.1038/s41598-021-92194-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of nerves inside the fascial tissue: (A) Floating thoracolumbar fascia stained with anti-S100 antibody and ematoxylin: the nervous structures are S100 positive (n: small nerve, arrows indicate single nerve fibers), whereas blood vessels are not stained (v: vessel; *: endothelial cells; a: adipocytes). (B) Semithin section of thoracolumbar fascia, whose boxes show nerve structures in the midst of collagen bundles of the fascial layers. (C) and (D): TEM images of a small nerve fiber in the inner layer (C) or in the outer layer (D) of the TLF, with both myelinc and unmyelinic axons. m: muscle; TLF: thoracolumbar fascia; mAx: myelinic axon; unAx: unmyelinic axon. Scale bars: (A) and (B) 30 µm; (C) 3 µm; (D) 2 µm.