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. 2021 Jun 16;12:3653. doi: 10.1038/s41467-021-23939-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Coronal sections of mouse somatosensory cortex immunostained for pS6 (green) and PV (red) at P18 (a1) and P26 (a2). b Number of PV cells expressing detectable levels of pS6 increases during the second to fourth postnatal week (Welch’s t-test, *p = 0.0152). Number of mice; P18, n = 4; P26, n = 3. c Mean pS6 intensity in individual PV cells is significantly higher at P26 than at P18 (Welch’s t-test, **p = 0.0061). Number of mice; P18, n = 10; P26 n = 7. d Coronal sections of mouse somatosensory cortex immunostained for pS6 (red) and NeuN (blue) at P18 (d1) and P26 (d2). e Percentage of colocalization of pS6 and NeuN is not significantly different between the two developmental ages (Welch’s t-test, p = 0.7663). Number of mice; P18, n = 4; P26, n = 3. Scale bars in a1-a2, d1-d2, 75 µm. Data represent mean ± SEM. Source data are provided as a Source Data file.