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. 2021 Jun 16;12:3669. doi: 10.1038/s41467-021-23925-z

Fig. 6. PGC-1α restores barrier function and serves as a novel antiviral.

Fig. 6

a SR18292 (PGC-1α inhibitor) dose response on oligomycin A-treated ALI cultures measuring FITC-dextran (3–5 kDa) flux 12 h post infection (top). Loss of oligomycin A barrier restoration by 100 μM SR18292 (bottom). Data are represented as boxplots and individual HBE donor lines and analyzed by one-way ANOVA with Dunnett multiple comparisons: **p = 0.005, n = 5 donors. b PPARGC1A mRNA expression by treatment with 3 μM oligomycin A in the presence of SR18292 at 12 h post infection, measured by RT-PCR. Data are represented as boxplots and analyzed by one-way ANOVA with Dunnett multiple comparisons: *p = 0.02, n = 5 donors. c Matched HRV-C15 intracellular RNA copy number at 12 h post infection, quantified by RT-PCR. Dashed line represents RT-PCR detection limit. There was no detectable HRV-C15 in noninfected ALI cultures. Data are represented as boxplots and analyzed by one-way ANOVA with Dunnett multiple comparisons: ***p = 0.0002, n = 5 donors. d ZLN005 (PGC-1α activator) dose response in HRV-C15-infected ALI cultures measuring FITC-dextran (3–5 kDa) flux at 12 h post infection (top). HRV-C15 barrier recovery by 100 μM ZLN005 at 12 h post infection (bottom). Data represented as boxplots and individual donor lines and analyzed by one-way ANOVA with Holm–Sidak multiple comparisons: ***p = 0.0008, **p = 0.03, n = 6 donors. e PPARGC1A mRNA expression in response to ZLN005 treatment, measured at 12 h post infection. Data are represented as boxplots and analyzed by one-way ANOVA with Dunnett multiple comparisons: **p = 0.002, n = 6 donors. f Matched HRV-C15 intracellular RNA copy number, quantified by RT-PCR. Dashed line represents RT-PCR detection limit. There was no detectable HRV-C15 in noninfected cultures. Data are represented as boxplots and analyzed by one-way ANOVA with Holm–Sidak multiple comparisons: **p = 0.001, **p = 0.005, n = 6 donors. Data represented as boxplots indicate the median (center line), upper and lower box bounds (IQR = first and third quartiles), and whiskers (min and max values), with individual donor data points superimposed onto the boxplot.