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. 2020 Aug 18;42(7):1124–1138. doi: 10.1038/s41401-020-0497-0

Fig. 1. Effect of BAH on collagen gel contraction and HIF activity.

Fig. 1

a Chemical structures of betulinic acid and BAH. b Images of contracted gel matrices in response to 12 h of BAH exposure. c Gel surface area quantified in terms of total pixel number using ImageJ. d NIH-3T3 fibroblasts stably transfected with a luciferase-HRE (NIH3T3-EPO-luc) were stimulated with the indicated concentrations of BAH or DMOG. e Correlation between HIF activity induced by BAH and collagen gel contraction. f HIF-1α protein expression in NIH-3T3-Epo-luc cells after 6 h of treatment with BAH and DMOG. Values are expressed as the mean ± SD. *P < 0.05 and ** P < 0.01 versus control group; significance was determined by one-way ANOVA followed by Dunnett’s post hoc test.