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. 2021 Jun 16;11:12630. doi: 10.1038/s41598-021-91572-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Biocompatibility towards HDFA cells. (a, b). Primary human fibroblast (HDFa cells) viability in the presence of mycelia extract (a) or semi-contact (b) as measured by MTS assay after 24 h. The reported values indicate the dilutions prepared starting from a stock solution of 20 mg of mycelium in 1 mL of Medium 106 containing growth factors. Data are expressed as average ± standard error and a p < 0.01 (**) was considered as significant. (c, d). Confocal images of control cells plated onto glass substrates and treated with Medium 106 containing growth factors (c), or in semi-contact with a 5 mg-piece of P. ostreatus (d).