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. 2021 Jun 16;11:12630. doi: 10.1038/s41598-021-91572-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal (a) and SEM micrographs (c, e) of HDFa cells grown for 48 h onto a FN-coated glass coverslips. Confocal (b) and SEM micrographs (d, f) of HDFa cells grown for 48 h onto a FN-coated P. ostreatus substrate. Nuclei are stained with DAPI (visible in blue), while the actin fibers are stained with Alexa Fluor Phalloidin 488 (highlighted in green). Filopodia extending from the cytoplasm of the attached cells are indicated with yellow arrows. (g–j) Effect of the scaffold coating onto primary cell growth. HDFa cells are seeded onto P. ostreatus scaffolds, either coated with fibronectin (g, h) or uncoated (i, j). Filopodia extending out of the attached cells are highlighted with yellow arrows.