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. 2021 Jun 16;11:12726. doi: 10.1038/s41598-021-92125-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Southern blot analysis of mtDNA molecules from the parental strains S. cerevisiae CCY 21-4-96 and S. paradoxus CBS 2908 and hybrid 4-R3 (72 K). HinfI restriction analysis of mtDNAs is at the left, while the autoradiograms showing hybridizations with the cox3, rns, and trnM probes are to the right. The cox3 and rns probes were ³²P-labeled PCR products of the corresponding genes. The trnM probe was an oligonucleotide of which the 5′ end was labeled with γ³²P-ATP. The arrows on the trnM picture show the S. cerevisiae band that is present in the 4-R3 (72 K) hybrid.