Fig. 4.

Selection of strain UV_6 for further analyses.

A) Analysis of the mean mRuby2 fluorescence in transformants derived from strain CC-1690*** (grey and coloured bars) or UVM4 (black bars). Fluorescence readings were normalized to chlorophyll absorption determined at 680 nm. Values are given relative to the normalized fluorescence recorded for the wildtype (set to 1). Error bars indicate the standard error derived from three biological replicates (n = 3).

B) Cadaverine titers reached in different UV strain-derived transformants after 7 days of cultivation in TAP medium containing acetate for mixotrophic growth.

C) Biomass dry weight (left y-axis, lines) and cadaverine titers (right y-axis, bars) determined for strains UV_2 (red), UV_10 (blue) and UV_6 (green) after 14 days of cultivation in 6xP medium for high cell density cultivation under photoautotrophic conditions.