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. 2021 Jun 16;11:12626. doi: 10.1038/s41598-021-92147-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AK5 methylation status and expression levels in CRC tissues and adjacent normal tissues. The AK5 methylation status in 105 paired CRC and adjacent normal tissues and eleven normal tissues was assessed using methylation bead chip array and qMSP. (a) The chip array results showed that AK5 is hypermethylated in 21 CRC tissues compared to adjacent normal tissues. (b) The AK5 gene is dramatically and significantly hypermethylated in 105 adjacent normal tissues and 105 CRC tissues compared to normal tissues. (c) AK5 mRNA expression decreased in the adjacent normal tissues compared to the normal tissues and decreased in the CRC tissues compared to the adjacent normal tissues, but the effect was not statistically significant. (d) AK5 protein expression decreased in CRC tissues compared to adjacent normal tissues. (e) AK5 hypermethylation was significant in CRC tissues compared to paired adjacent normal tissues in 49 CRC tissues with a cut off value of 40%. (f) In 49 CRCs, AK5 mRNA expression significantly decreased in CRC compared to 49 paired adjacent normal tissues. Full-length bolts images or original gel images of AK5 protein expression are presented in Figure S5a. * P-values of < 0.05 were considered statistically significant. PMR percentage of methylated reference, AN adjacent normal tissue, T colorectal cancer tissue, N normal colon tissue, IB immunoblot.