Figure 1.

The FVIII Residue Interaction Network (RIN). (a) Each residue of the FVIII structure is represented as a node in the RIN. Two nodes are connected if either their main- or side-chains are close to each other (less than ~ 5 Å). Note that the RIN does not keep the three dimensional positional information of the domains or residues. The centrality of each node can be calculated based on the number of residues they interact (degree), whether they serve as bridges for groups of residues that would not be connected otherwise (betweenness), and are located in a position that requires few ‘steps’ to reach every other node in the network (closeness). Image created using the structure 2R7E (Ref.³) and Chimera 1.14 (Ref.⁶²). (b) Several measures display a moderate to strong Pearson correlation to each other, despite being calculated using different underlying principles.