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. 2021 Jun 5;45:102033. doi: 10.1016/j.redox.2021.102033

Fig. 6.

Fig. 6

SAHH inhibition increased TXNIP by inhibiting EZH2 expression. (A) Protein expression of EZH2 was assayed by Western blot in kidney tissues. (B) Representative immunofluorescence images and quantitation of EZH2 expression in kidney tissues sections. Scale bar, 20 μm. (C) Protein expression of EZH2 and H3K27me3 were assayed by Western blot in podocytes. (D) Protein expression of EZH2 and H3K27me3 were assayed by Western blot in podocytes treated with AdEZH2 or Adctrl. (E) Protein expression of TXNIP was assayed by Western blot in podocytes treated with ADA (20 μmol/L) with or without AdEZH2 infection. (F) Intracellular ROS production was measured by DCF staining in podocytes treated with ADA (20 μmol/L) with or without AdEZH2 infection. Values are mean ± SEM; n = 3 for in vitro, 6–8 for in vivo assays, *P < 0.05 vs. control or Adctrl group; #P < 0.05 vs. ADA group (determined by one-way ANOVA or unpaired Student's t-test).