Fig. 9.

Knockdown of SAHH increases oxidative stress and inflammation and diabetic nephropathy. (A) The basic characteristics and plasma methionine metabolites levels of SAHH^-/- and wild type mice, (B–C) Representative images and quantitation of SAHH immunofluorescence staining, PAS staining, TEM examination, WT1, F-actin, and Masson staining, (D) The levels of caspase-1 activity and IL-1β production, urine albumin and creatinine, and urinary levels of 8-OHdG was measured by commercial kits. (E) Representative images and quantitation of F-actin staining and ROS levels in podocytes treated with SAHH shRNA. (F) Protein expression of SAHH, EZH2, EGR1, TXNIP, and NLRP3 was assayed by Western blot in vitro and in vivo. Values are mean ± SEM; n = 3 for in vitro, 6–8 for in vivo assays, *P < 0.05 vs. WT (determined by unpaired Student's t-test).