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. 2020 Nov 13;8(4):531–544. doi: 10.1016/j.gendis.2020.11.004

Figure 2.

Figure 2

Comprehensive analysis of the 14 types of BMPs in regulating glucose metabolism in hepatocytes. The MPH cells were infected with 14 Ad-Bs and Ad-GFP respectively for 3 days. PAS staining was used to assess hepatic glycogen accumulation (magnification, x200) (A) Ad-siB4 and Ad-RFP infected the MPH cells for 3 days, PAS staining was used to assess the hepatic glycogen accumulation (magnification, x200) (B) Ad-B4, Ad-GFP, Ad-siB4 and Ad-RFP infected the MPH cells for 12h, 24h, 36h, 48h and 72h, total glucose content in hepatocytes were measured by absorbance reading at 5 time points. “∗∗” P < 0.01 Ad-B4 group vs. Ad-GFP group, Ad-siB4 group vs. Ad-RFP group (C) Ad-B4, Ad-GFP, Ad-siB4 and Ad-RFP infected the MPH cells for 36h and 72h, respectively, total RNA was isolated and TqPCR analysis was carried out to detect the expression of the genes that govern glycogen synthesis, glucose metabolism, glycolysis and gluconeogenesis respectively. Relative expression was calculated by dividing the relative expression values (i.e., gene/Gapdh) in “∗∗” P < 0.01, “∗” P < 0.05, Ad-B4 group vs. Ad-GFP group, Ad-siB4 group vs. Ad-RFP group (D). Each assay condition was done in triplicate. Representative images are shown.