Table 6.

Summary of PCR oligonucleotides used for detection of microsporidia (Encephalitozoon intestinalis, Encephalitozoon cuniculi, Enterocytozoon bieneusi, Encephalitozoon hellem) and Toxocara spp. in fresh produce.

Type of PCR	Target gene	Sequence	Amplicon size (bp)	Note	Reference
Nested	18S rRNA (Encephalitozoon spp., E. bieneusi)	1st: PMicF: 5′- GGTTGATTCTGCCTGACG -3′; PMicR: 5′ − CTTGCGAGC(G/A)TACTATCC -3′	779	Positive samples sequenced.	Javanmard et al. (2018)
		2nd: EnbF: 5′- GGTAATTTGGTCTCTGTGTG -3′; EnbR: 5′- CTACACTCCCTATCCGTTC -3′	440
		EncepF: 5′- AGTACGATGATTTGDTTG-3′; EncepR: 5′- ACATAAGTCCAAGARCACA -3′	530
conventional	ITS (E. bieneusi)	AL4038: 5′-AGGGATGAAGAGCTTCGGCTCTG-3′; AL4040: 5′-AATATCCCTAATACAGGATCACT-3′	392	Positive samples sequenced.	Li et al. (2019)
conventional	nad2 (Toxocara spp.)	F: 5′-GGAGTTGTTTAAGTTGGATGG-3′; R: 5′ -AGAACTCCGCCTTATCAAGACGAC-3´	227		Guggisberg et al. (2020)