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. 2021 Jun 3;12:690181. doi: 10.3389/fphar.2021.690181

TABLE 1.

Sequences of primers.

Gene Forward primer (5′–3′) Reverse primer (5′–3′)
CH25H ACG​GAG​CAA​AGT​TGC​AGT​TAA GGA​GGA​CCA​CTC​AGG​TTA​CGA
CYP7B1 GAA​GTC​CTG​CGT​GAC​GAA​AT CCT​CAG​AAC​CTC​AAG​AAT​AGC​G
RORα CTA​CCA​GAA​CAA​GCA​GAG​A CGAACTCCACCACATACT
MMP3 GAG​GAC​AAA​TTC​TGG​AGA​TTT​GAT​G GTG​AAG​ATC​CGC​TGA​AGA​AGT​AAA​G
MMP13 CAG​ACA​GCA​AGA​ATA​AAG​AC CAA​CAT​AAG​CAC​AGT​GTA​AC
SOX9 ACTTGCACAACGCCGAG CTG​GTA​CTT​GTA​ATC​CGG​GTG
COL2α1 TCC​TAA​GGG​TGC​CAA​TGG​TGA GGA​CCA​ACT​TTG​CCT​TGA​GGA​C
Aggrecan TCC​GCT​GGT​CTG​ATG​GAC​AC CCA​GAT​CAT​CAC​TAC​GCA​GTC​CTC
Adamts4 GGT​GGC​AGA​TGA​CAA​GAT​G AGT​CGT​TCG​GAG​GGT​TTA​G
Adamts5 GCA​TCA​TCG​GCT​CAA​AGC​TAC​A TCA​GGG​ATC​CTC​ACA​ACG​TCA​G
GAPDH TGA​CCT​CAA​CTA​CAT​GGT​CTA​CA CTT​CCC​ATT​CTC​GGC​CTT​G
miR-10a-3p CGC​GCA​AAT​TCG​TAT​CTA​GG AGT​GCA​GGG​TCC​GAG​GTA​TT
miR-10a-3p RT GTC​GTA​TCC​AGT​GCA​GGG​TCC​GAG​GTA​TTC​GCA​CTG​GAT​ACG​ACT​ATT​CC
U6 CTCGCTTCGGCAGCACA AAC​GCT​TCA​CGA​ATT​TGC​GT
U6 RT GTC​GTA​TCC​AGT​GCA​GGG​TCC​GAG​GTA​TTC​GCA​CTG​GAT​ACG​ACA​AAA​TA