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L929 cells cultured with (A) non-sterilised, (B) PAA-treated, (C) gamma irradiated or (D) E Beam treated decellularised nerve. (E) Surgical closure strip and (F) cyanoacrylate adhesive were used as negative and positive controls, respectively. The location of the tissue samples is indicated by the dark shadow regions. Cells were stained using Giemsa’s stain. Images were acquired using a ×10 objective, scale bars = 100 μm.
