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. 2021 Jun 3;12:653901. doi: 10.3389/fphar.2021.653901

FIGURE 8.

FIGURE 8

Adenosine 5'-monophosphate–activated protein kinase (AMPK) silencing the reversed anti-hypertrophy effect of BCW in neonatal rat cardiomyocytes induced by ISO. (A) mRNA and (B–C) protein expression of AMPK in cardiomyocytes transfected with three different siRNAs for AMPK or negative control (NC) for 48 h. (D–F) Protein expression of AMPK and PPAR-α in primary neonatal rat cardiomyocytes infected with or without AMPK siRNA or the NC for 48 h, or treated with or without 10 μM ISO for 24 h. (G) Cell surface area measured by rhodamine phalloidin staining (red for rhodamine phalloidin and blue for DAPI) and in primary neonatal rat cardiomyocytes infected with or without AMPK siRNA or the NC for 48 h, or treated with or without 10 μM ISO for 24 h (observed at 200× magnification). (H) The mRNA expressions of the ANF, BNP, and β-MHC determined by qRT-PCR in primary neonatal rat cardiomyocytes infected with or without AMPK siRNA or the NC for 48 h, or treated with or without 10 μM ISO for 24 h (observed at 200× magnification). Data were normalized by GADPH for mRNA expression and protein expression and presented as mean ± SE. *p < 0.05 vs. control; # p < 0.05 vs. ISO, $ p < 0.05 vs. ISO + BCW + NC n = 3.