Fig. 3.

Proportion of reads in sequence libraries constructed from 10 pg to 1‍ ‍ng dsRNA with the U2-DNA-5P3H adaptor. Libraries were generated under either conventional (PCR amplification with 2.5‍ ‍mM Mg²⁺ and cDNA purification performed once) or revised (PCR amplification with 1.5‍ ‍mM Mg²⁺ and cDNA purification performed twice) conditions. All libraries were prepared with 25 cycles of cDNA amplification, except for the library generated from 10 pg dsRNA under conventional conditions (35 cycles).