Table 1.

Assembly statistics for cDNA libraries constructed with 5 types of oligonucleotide adaptors.

	U2-DNA-5P3P	U2-DNA-5P3H	U2-DNA-5P3am	U2-RNA-5P3H	U2-DNA-5P3H/ 5OH3H
cDNA amplification	25 cycles
Sampled reads	0.8 M	0.8 M	0.8 M	0.8 M	0.8 M
Prokaryotic and eukaryotic reads (%)	0.93	0.76	0.89	1.14	0.89
Assembly
Number of contigs	366	459	445	295	343
Total size (kb)	479.8	649.7	610.8	382.7	474.7
Average length (bp)	1,310	1,415	1,372	1,297	1,383
N50 (bp)	1,587	1,858	1,637	1,588	1,725
GC content (%)	48.3	48.5	48.8	49.3	48.5
cDNA amplification	35 cycles
Sampled reads	0.8 M	0.8 M	0.8 M	0.8 M
Prokaryotic and eukaryotic reads (%)	0.5	0.4	0.53	0.72
Assembly
Number of contigs	376	422	420	337
Total size (kb)	487.7	583	564.5	402
Average length (bp)	1,296	1,381	1,344	1,193
N50 (bp)	1,573	1,656	1,542	1,293
GC content (%)	48.3	48.5	48.4	48.8