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Assembly statistics for cDNA libraries constructed from 10 pg to 1 ng dsRNA and 25 cycles of cDNA amplification.
| 1 ng | 100 pg | 10 pg | |
|---|---|---|---|
| Sampled reads | 0.8 M | 0.8 M | 0.8 M |
| Prokaryotic and eukaryotic reads (%) | 0.22 | 0.57 | 0.80 |
| Assembly | |||
| Number of contigs | 474 | 456 | 235 |
| Total size (kb) | 741.2 | 661.7 | 269.9 |
| Average length (bp) | 1,563 | 1,451 | 1,148 |
| N50 (bp) | 2,019 | 1,756 | 1,348 |
| GC content (%) | 48.2 | 48.8 | 49.2 |
