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. 2021 Jun 3;12:690535. doi: 10.3389/fphar.2021.690535

FIGURE 2.

FIGURE 2

FGF1ΔHBS suppressed renal fibrosis and TGF-β1 signaling in db/db mice. (A) Real-time PCR analysis of Acta2, Fn1, and Col 4 mRNA expression. (B) Expression levels of α-SMA, COL 1, and COL 4 as determined by western blot analysis and quantitation using ImageJ. (C) Real-time PCR analysis of TGF-β1 mRNA expression levels. (D) Representative images of TGF-β1 immunohistochemical staining of renal tissues and quantitation using ImageJ. (E) Representative images of phosphorylated SMAD3 immunohistochemical staining of renal tissues and quantitation using ImageJ. (F) Expression levels of phosphorylated SMAD3, SMAD3, and TGF-β1 as determined by western blot analysis and quantitation using ImageJ. Data are presented as the mean ± SEM (n = 6); Panels A and B, *p < 0.05, ***p < 0.001 vs. db/m; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. db/db; Panels C–F, **p < 0.01, ***p < 0.001.