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. 2021 Jun 3;12:690535. doi: 10.3389/fphar.2021.690535

FIGURE 3.

FIGURE 3

PPARγ mediated the protective effects of FGF1ΔHBS on podocyte EMT in diabetic conditions. (A) Real-time PCR analysis of PPARγ mRNA expression. (B) Representative images and quantitation of immunofluorescence staining for PPARγ. (C) Expression levels of PPARγ as determined by western blot analysis and quantitation using ImageJ. (D) Phosphorylation levels of SMAD3 and protein expression of PPARγ as determined by western blot analysis and quantitation using ImageJ. (E) Real-time PCR analysis of TGF-β1, Fn1, Col 4, and Acta2 mRNA expression. (F) Representative images and quantitation of immunofluorescence staining of SMAD3 and PPARγ. In panels (A–C), data are presented as the mean ± SEM (n = 6). In panels (D–F), data from three independent measurements are presented as the mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.