FIGURE 2.

IBSP expression was upregulated in various independent datasets, clinical specimens, and CRC cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: nonsignificant. (A) and (B) IBSP expression was specifically higher in the primary CRC and metastatic CRC, respectively, compared with normal colonic mucosa and primary CRC in GSE41258 dataset. (C) IBSP expression was specifically higher in CRC patients with metastatic recurrence, compared with CRC patients with non‐recurrence in GSE21510 dataset. (D) IBSP expression was specifically higher in the consensus molecular subtype 4 CRC patients (CMS4, with high risk of recurrence), compared with the CMS1, 2,3 CRC patients (with low risk of recurrence) in GSE39582 dataset. (E) The mRNA level of IBSP was specifically higher in the primary CRC, compared with normal colorectal mucosa in clinical specimens. (F) The mRNA level of IBSP was specifically increased in the CRC liver metastatic tumors, compared with normal liver tissues in clinical specimens. (G and H) The mRNA and protein level of IBSP were significantly increased in several CRC cell lines compared to human normal colorectal epithelial FHC cells