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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: J Endocrinol. 2010 Jun 3;206(3):317–325. doi: 10.1677/JOE-10-0075

Figure 2.

Figure 2

Catalytic activity of recombinant zebrafish CYPs co-expressed with human NADPH–CYP reductase. Data are shown for the metabolism of 7-ethoxyresorufin (7-ER) and 7-benzyloxy-4-(trifluoromethyl)coumarin (BFC), typical CYP1 and CYP3A substrates in mammals (Lake et al. 2009) and fish (Kashiwada et al. 2005). 7-ER and BFC are metabolized to resorufin and HFC respectively. Results are mean±s.e.m., for duplicate reactions. Catalytic activity was below the limits of detection for CYP3A65 (7-ER) and CYP1D1 (BFC). Values represent Mean±s.e.m. of reactions done in duplicate. *Resorufin was detected in CYP1D1 at a rate of 24±5 pmol/nmol P450/min.