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. 2021 Jun 11;2(2):100594. doi: 10.1016/j.xpro.2021.100594
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

APC Rat monoclonal anti-PDGFRα BioLegend Cat#135907; RRID: AB_2043969
APC Rat IgG2a BioLegend Cat#400511; RRID: AB_1952838

Chemicals, peptides, and recombinant proteins

Naphthalene Sigma-Aldrich Cat#84679
Tamoxifen Sigma-Aldrich Cat#T5648
Insulin-Transferrin-Selenium Biozym Cat#41400-045
l-Glutamine Gibco Cat#11539876
PBS Gibco Cat#10010023
EDTA Roth Cat#6764.1
Matrigel matrix Corning Cat#356231
DMEM Gibco Cat#12491015
αMEM Gibco Cat#41061029
Dispase Corning Cat#354235
Collagenase IV Gibco Cat # 17104019
DNase I Serva Cat#18535.01
Heparin Stemcell Technologies Cat#07980
Y-27632 ROCK inhibitor Stemcell Technologies Cat#72304
rmFGF10 R&D Cat#6224-FG-025/CF
rmHGF R&D Cat#2207-HG-025/CF
Ethanol Merck Cat#32305
Hepes Gibco Cat#12509079
Matrigel matrix Corning Cat #356231
HBSS Gibco Cat#14175095
FCS Cell Biologics Cat#6912
Pen/Strep/L-Glut Merck Cat#G6784
Corn oil Sigma-Aldrich Cat#C8267-500ML

Experimental models: organisms/strains

Mouse: Tg(Acta2-cre/ERT2)12Pcn Pierre Chambon (Wendling et al., 2009) MGI: 3831907
Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson Laboratory RRID: IMSR_JAX:007905
Mouse: B6N.129S6(Cg)-Scgb1a1tm1(cre/ERT)Blh/J Jackson Laboratory RRID: IMSR_JAX:016225
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J Jackson Laboratory RRID: IMSR_JAX:007914

Software

FlowJo FlowJo, LLC https://www.flowjo.com/solutions/flowjo
RRID: SCR_008520

Other

5 mL polypropylene round-bottom tube Corning Cat#352063
5 mL polystyrene round-bottom tube with cell-strainer cap Corning Cat#352235
60-mm glass petri dishes Sigma-Aldrich Cat#CLS7016560
Scalpel blades Fisher Scientific Cat# S95937A
Cell culture inserts 0.4 μm, 12 mm diameter Millicell Cat#PICM01250
24-Well cell culture plare Greiner Bio-One Cat#662160
Cell strainer 100 μm Sarstedt Cat#833945100
Cell strainer 70 μm Sarstedt Cat#833945070
0.2 μm filter Sarstedt Cat#831826001
BD FACSAriaTMIII Cell Sorter BD Biosciences Cat#648282
GentleMACS® Dissociator MACS Miltenyi Biotec Cat#130-093-235
Laminar hood Class II Heraeus Cat#50033851
Mettler-Toledo™ pH meter Sigma-Aldrich Cat#MT30130864