REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
APC Rat monoclonal anti-PDGFRα | BioLegend | Cat#135907; RRID: AB_2043969 |
APC Rat IgG2a | BioLegend | Cat#400511; RRID: AB_1952838 |
Chemicals, peptides, and recombinant proteins | ||
Naphthalene | Sigma-Aldrich | Cat#84679 |
Tamoxifen | Sigma-Aldrich | Cat#T5648 |
Insulin-Transferrin-Selenium | Biozym | Cat#41400-045 |
l-Glutamine | Gibco | Cat#11539876 |
PBS | Gibco | Cat#10010023 |
EDTA | Roth | Cat#6764.1 |
Matrigel matrix | Corning | Cat#356231 |
DMEM | Gibco | Cat#12491015 |
αMEM | Gibco | Cat#41061029 |
Dispase | Corning | Cat#354235 |
Collagenase IV | Gibco | Cat # 17104019 |
DNase I | Serva | Cat#18535.01 |
Heparin | Stemcell Technologies | Cat#07980 |
Y-27632 ROCK inhibitor | Stemcell Technologies | Cat#72304 |
rmFGF10 | R&D | Cat#6224-FG-025/CF |
rmHGF | R&D | Cat#2207-HG-025/CF |
Ethanol | Merck | Cat#32305 |
Hepes | Gibco | Cat#12509079 |
Matrigel matrix | Corning | Cat #356231 |
HBSS | Gibco | Cat#14175095 |
FCS | Cell Biologics | Cat#6912 |
Pen/Strep/L-Glut | Merck | Cat#G6784 |
Corn oil | Sigma-Aldrich | Cat#C8267-500ML |
Experimental models: organisms/strains | ||
Mouse: Tg(Acta2-cre/ERT2)12Pcn | Pierre Chambon (Wendling et al., 2009) | MGI: 3831907 |
Mouse: B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Jackson Laboratory | RRID: IMSR_JAX:007905 |
Mouse: B6N.129S6(Cg)-Scgb1a1tm1(cre/ERT)Blh/J | Jackson Laboratory | RRID: IMSR_JAX:016225 |
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Jackson Laboratory | RRID: IMSR_JAX:007914 |
Software | ||
FlowJo | FlowJo, LLC |
https://www.flowjo.com/solutions/flowjo RRID: SCR_008520 |
Other | ||
5 mL polypropylene round-bottom tube | Corning | Cat#352063 |
5 mL polystyrene round-bottom tube with cell-strainer cap | Corning | Cat#352235 |
60-mm glass petri dishes | Sigma-Aldrich | Cat#CLS7016560 |
Scalpel blades | Fisher Scientific | Cat# S95937A |
Cell culture inserts 0.4 μm, 12 mm diameter | Millicell | Cat#PICM01250 |
24-Well cell culture plare | Greiner Bio-One | Cat#662160 |
Cell strainer 100 μm | Sarstedt | Cat#833945100 |
Cell strainer 70 μm | Sarstedt | Cat#833945070 |
0.2 μm filter | Sarstedt | Cat#831826001 |
BD FACSAriaTMIII Cell Sorter | BD Biosciences | Cat#648282 |
GentleMACS® Dissociator | MACS Miltenyi Biotec | Cat#130-093-235 |
Laminar hood Class II | Heraeus | Cat#50033851 |
Mettler-Toledo™ pH meter | Sigma-Aldrich | Cat#MT30130864 |