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Preparation of UCB MNCs from human cord blood samples
(A) Fresh UCB sample diluted with isolation buffer was layered onto Ficoll Paque Plus without disturbing the interface.
(B) Separation of the four fractions after density gradient centrifugation. From top to bottom: plasma + platelets, UCB MNCs, Ficoll Paque Plus, and granulocytes + erythrocytes.
(C) Assembly of the MACS MS column with the MACS Separator and the MACS MultiStand.
See also Methods video S1.
