Figure 3.

Hydration of sensor cartridge and a typical plate map of Glycolytic Rate Assay

(A) (Top) Image of the Seahorse XFe96 sensor cartridge set with its lid, sensor cartridge, and utility plate labeled. (Bottom) When filling the utility plate with calibrant to hydrate the sensor cartridge, put the sensor cartridge with the lid upside down to avoid sensors touching work surface.

(B) A typical plate map with 8 wells in a row assigned to each experimental group in the Glycolytic Rate Assay. Similarly, a row of 8 wells are assigned to background control group and only assay medium without cells will be added to these wells.