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A typical running protocol for the Glycolytic Rate Assay
This running protocol includes an initialization measurement stage (calibration + equilibration), a basal glycolysis measurement step (3 measurement cycles of 6 min each), a compensatory glycolysis measurement step (after addition of Rot/AA; 3 measurement cycles of 6 min each), and a residual background acidification measurement stage (after addition of 2-DG; 3 measurement cycles of 6 min each).
