Figure 6.

Good and bad results from the Glycolytic Rate Assay

(A) A typical good result from Glycolytic Rate Assay demonstrating a 3-stage ECAR (extracellular acidification rate; corresponding to glycolytic activity) curve: the basal stage, the compensatory stage, and the post 2-DG stage. The glycolytic rates were calculated by the Seahorse XF Glycolytic Rate Assay Report Generator. The assay was performed with U937 cells and used as an example to show the expected pattern of Glycolytic Rate Assay.

(B) The number of cells seeded per well will directly affect the ECAR and OCR values measured during the assay. The assay was performed with U937 cells and used as an example to show the impact of different cell densities on the result.

(C) A bad result from Glycolytic Rate Assay due to incorrect or uneven loading of assay compounds into the injection ports. The assay was performed with U937 cells and used as an example to show the impact of bad loading technique on the results.

(D) A bad result from Glycolytic Rate Assay due to the use of assay compounds that are ineffective to inhibit mitochondrial respiration or glycolysis. The assay was performed with U937 cells and used as an example to show the impact of using old compounds on the result.

Data are represented as mean ± SD.