Transfection complex
Reagent	Amount for 6-well plate (1 well)	Amount for 6 cm dish
pMD2.G	0.5 μg	1.25 μg
pMDLg/PRRE	0.3 μg	0.75 μg
pRSV-Rev	0.7 μg	2.1 μg
pLKO.1 shRNA for target gene	0.6 μg	1.8 μg
Diluent (Opti-MEM I Reduced Serum Medium)	0.2 mL	0.5 mL