SFEM complete medium

Reagent	Stock concentration	Final concentration	Amount
SFEM medium	n/a	n/a	49.4 mL
SCF	1000 μg/mL (100 μg/mL in the cytokine mixture)	100 ng/mL	50 μL of cytokine mixture
IL-3	100 μg/mL (10 μg/mL in the cytokine mixture)	10 ng/mL
IL-6	100 μg/mL (10 μg/mL in the cytokine mixture)	10 ng/mL
Penicillin-streptomycin	10,000 U/mL	100 U/mL	500 μL
Plasmocin prophylactic	2.5 mg/mL	2.5 μg/mL	50 μL
Total	n/a	n/a	50 mL

Prepare freshly, do not store.