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DMEM complete medium
| Reagent | Stock concentration | Final concentration | Amount |
|---|---|---|---|
| DMEM, high glucose, with pyruvate | n/a | n/a | 500.0 mL |
| FBS | n/a | 10% (vol/vol) | 56.0 mL |
| Penicillin-streptomycin | 10,000 U/mL | 100 U/mL | 5.6 mL |
| Plasmocin prophylactic | 2.5 mg/mL | 2.5 μg/mL | 562 μL |
| Total | n/a | n/a | 562.2 mL |
The DMEM complete medium can be stored for up to 3 months at 2°C–8°C.
