Freezing medium
| Reagent | Stock concentration | Final concentration | Amount |
|---|---|---|---|
| FBS | n/a | 90% (vol/vol) | 45 mL |
| Dimethyl sulfoxide (DMSO) | n/a | 10% (vol/vol) | 5 mL |
| Total | n/a | n/a | 50 mL |
The freezing medium can be stored for up to 6 months at 2°C–8°C.
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