REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD34 FITC (4H11)	eBioscience	Cat# 11-0349-42; RRID: AB_1518732
Biological samples
Fresh human umbilical cord blood	StemCyte	https://www.stemcyte.com/
Chemicals, peptides, and recombinant proteins
Puromycin Dihydrochloride	Sigma-Aldrich	Cat# P8833; CAS: 58-58-2
Bovine Serum Albumin solution	Sigma-Aldrich	Cat# A7034; CAS: 9048-46-8
Recombinant Human IL-6	PeproTech	Cat# 200-06
Recombinant Human IL-3	PeproTech	Cat# 200-03
Recombinant Human SCF	PeproTech	Cat# 300-07
L-Glutamine (200 mM)	Thermo Fisher Scientific	Cat# 25030-081
Sodium Pyruvate (100 mM)	Thermo Fisher Scientific	Cat# 11360-070
Penicillin Streptomycin	Thermo Fisher Scientific	Cat# 15-140-122
Plasmocin prophylactic	InvivoGen	Cat# ant-mpp
M-PER Mammalian Protein Extraction Reagent	Thermo Fisher Scientific	Cat# 78501
Ficoll Paque Plus	GE Healthcare	Cat# 17-1440-02
DMEM, high glucose, with pyruvate	Life Technologies	Cat# 11995-073
X-tremeGENE HP DNA Transfection Reagent	Sigma-Aldrich	Cat# 6366546001
RetroNectin® Recombinant Human Fibronectin Fragment	Takara Bio Inc.	Cat# T100B
Trypsin-EDTA 0.05%	Fisher Scientific	Cat# 25-300-120
Fetal bovine serum (FBS)	Gemini Bio-Products	Cat# 100-106
Dimethyl Sulfoxide (DMSO)	Fisher Bioreagents	Cat# BP231-100; CAS: 67-68-5
HEPES (1M)	Thermo Fisher Scientific	Cat# 15630080
Polybrene	Sigma-Aldrich	Cat# H9268; CAS: 28728-55-4
StemSpan SFEM medium	STEMCELL Technologies	Cat# 09650
UltraPure 0.5M EDTA, pH 8.0	Invitrogen	Cat# 15575020
Cell-Tak Cell and Tissue Adhesive	Corning	Cat# 354240
Seahorse XF RPMI Medium, pH 7.4	Agilent Technologies	Cat# 103576-100
Glucose Solution	Gibco	Cat# A2494001
Sodium bicarbonate	Sigma-Aldrich	Cat# S5761-500G; CAS: 144-55-8
Critical commercial assays
CD34 MicroBead Kit, human	Miltenyi Biotec	Cat# 130-046-702
Seahorse XF Glycolytic Rate Assay Kit	Agilent Technologies	Cat# 103344-100
Bio-Rad Protein (Bradford) Assay Dye Reagent	Bio-Rad	Cat# 5000006
Seahorse XFe96 FluxPak mini	Agilent Technologies	Cat# 102601-100
Experimental models: Cell lines
293T	ATCC	(CRL-3216); RRID:CVCL_0063
Recombinant DNA
pMD2.G	A gift from Dr. Didier Trono	Addgene plasmid # 12259; RRID: Addgene_12259
pMDLg/pRRE	(Dull et al., 1998)	Addgene plasmid # 12251; RRID: Addgene_12251
pRSV-Rev	(Dull et al., 1998)	Addgene plasmid # 12253; RRID: Addgene_12253
pLKO.1-shNS	(Stewart et al., 2003)	Addgene plasmid # 8453
pLKO.1-shFTO-1	Sigma-Aldrich	TRCN0000246247
pLKO.1-shFTO-2	Sigma-Aldrich	TRCN0000246249
Software and algorithms
GraphPad Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
Seahorse Wave	Agilent Technologies	https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-wave-desktop-software-740897
Seahorse XF Glycolytic Rate Assay Report Generators	Agilent Technologies	https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-xf-glycolytic-rate-assay-report-generators-740900
FlowJo v10	BD Life Sciences	https://www.flowjo.com/
Other
Falcon polystyrene microplates, non-treated	Corning	Cat# 351146
Seahorse XFe96 Analyzer	Agilent Technologies	n/a
Eppendorf Research plus 8-channel adjustable volume pipette, 30–300 μL	Eppendorf	Cat# 3125000052
Eppendorf Research plus 8-channel adjustable volume pipette, 10–100 μL	Eppendorf	Cat# 3125000036
Matrix Reagent Reservoirs	Thermo Scientific	Cat# 8094