Table 11.

Example pipetting scheme for qPCR detection

Reagent/component	Concentration per reaction	Volume per sample
DNase & RNase H2O		8.9 μL
10× PCR Buffer	1×	1.25 μL
MgCl2	1.5 mM	0.375 μL
dNTPs	0.2 mM	0.25 μL
Primer Forward	0.2 μM	0.25 μL
Primer Reverse	0.2 μM	0.25 μL
SYBR™ Green I Nucleic Acid Gel Stain	1×	0.125 μL
Platinum™ Taq DNA Polymerase	0.5 U	0.1 μL
DNA 1	max. 20 ng μL−1	1.0 μL
Total		12.5 μL

¹For fecal DNA solutions: use 20 ng μL⁻¹. For bacterial DNA standards, use the concentrations mentioned in Table 7.