Table 12.

qPCR reaction specifications

Steps	Temperature	Time	Cycles
Pre-denaturation	95°C	3 min	1
Denaturation	95°C	3 s	40
Annealing	55°C	20 s
Extension	68°C	20 s
Post-Extension	95°C	15 s	1
Pre-Melting-Curve Cooling Step	60°C	15 s
Melting Curve	60°C–95°C	Incremental increase of temperature by 0.3°C every 15 s until temperature reaches 95°C
Storage	20°C	→∞	1