Table 13.
Example Cq values of a qPCR run including standards for all primer pairs and DNA isolated from 1 sample (“Fecal 1”)
| Sample no | Sample type | Sample | Concentration | Forward primer | Reverse primer | Cq |
|---|---|---|---|---|---|---|
| 1 | Standard | AM culture | 20 ng μL−1 | AM F | AM R | 13.71 |
| 2 | Standard | AM culture | 2 ng μL−1 | AM F | AM R | 17.54 |
| 3 | Standard | AM culture | 0.4 ng μL−1 | AM F | AM R | 20.59 |
| 4 | Standard | AM culture | 0.01 ng μL−1 | AM F | AM R | 27.79 |
| 5 | Standard | BC culture | 20 ng μL−1 | BC F | BC R | 14.32 |
| 6 | Standard | BC culture | 2 ng μL−1 | BC F | BC R | 17.96 |
| 7 | Standard | BC culture | 0.4 ng μL−1 | BC F | BC R | 20.99 |
| 8 | Standard | BC culture | 0.01 ng μL−1 | BC F | BC R | 26.97 |
| 9 | Standard | BI culture | 20 ng μL−1 | BI F | BI R | 13.29 |
| 10 | Standard | BI culture | 2 ng μL−1 | BI F | BI R | 16.64 |
| 11 | Standard | BI culture | 0.4 ng μL−1 | BI F | BI R | 20.41 |
| 12 | Standard | BI culture | 0.01 ng μL−1 | BI F | BI R | 27.26 |
| 13 | Standard | BO culture | 20 ng μL−1 | BO F | BO R | 14.65 |
| 14 | Standard | BO culture | 2 ng μL−1 | BO F | BO R | 18.28 |
| 15 | Standard | BO culture | 0.4 ng μL−1 | BO F | BO R | 21.94 |
| 16 | Standard | BO culture | 0.01 ng μL−1 | BO F | BO R | 28.63 |
| 17 | Standard | BT culture | 20 ng μL−1 | BT F | BT R | 15.48 |
| 18 | Standard | BT culture | 2 ng μL−1 | BT F | BT R | 19.21 |
| 19 | Standard | BT culture | 0.4 ng μL−1 | BT F | BT R | 22.33 |
| 20 | Standard | BT culture | 0.01 ng μL−1 | BT F | BT R | 28.53 |
| 21 | Standard | BU culture | 20 ng μL−1 | BU F | BU R | 15.02 |
| 22 | Standard | BU culture | 2 ng μL−1 | BU F | BU R | 18.30 |
| 23 | Standard | BU culture | 0.4 ng μL−1 | BU F | BU R | 21.68 |
| 24 | Standard | BU culture | 0.01 ng μL−1 | BU F | BU R | 28.29 |
| 25 | Standard | CA culture | 20 ng μL−1 | CA F | CA R | 16.12 |
| 26 | Standard | CA culture | 2 ng μL−1 | CA F | CA R | 19.27 |
| 27 | Standard | CA culture | 0.4 ng μL−1 | CA F | CA R | 22.27 |
| 28 | Standard | CA culture | 0.01 ng μL−1 | CA F | CA R | 29.78 |
| 29 | Standard | CS culture | 20 ng μL−1 | CS F | CS R | 17.22 |
| 30 | Standard | CS culture | 2 ng μL−1 | CS F | CS R | 20.54 |
| 31 | Standard | CS culture | 0.4 ng μL−1 | CS F | CS R | 24.62 |
| 32 | Standard | CS culture | 0.01 ng μL−1 | CS F | CS R | 30.36 |
| 33 | Standard | DP culture | 20 ng μL−1 | DP F | DP R | 14.18 |
| 34 | Standard | DP culture | 2 ng μL−1 | DP F | DP R | 17.33 |
| 35 | Standard | DP culture | 0.4 ng μL−1 | DP F | DP R | 20.63 |
| 36 | Standard | DP culture | 0.01 ng μL−1 | DP F | DP R | 28.50 |
| 37 | Standard | EC culture | 20 ng μL−1 | EC F | EC R | 14.11 |
| 38 | Standard | EC culture | 2 ng μL−1 | EC F | EC R | 18.24 |
| 39 | Standard | EC culture | 0.4 ng μL−1 | EC F | EC R | 21.00 |
| 40 | Standard | EC culture | 0.01 ng μL−1 | EC F | EC R | 27.23 |
| 41 | Standard | ER culture | 20 ng μL−1 | ER F | ER R | 14.24 |
| 42 | Standard | ER culture | 2 ng μL−1 | ER F | ER R | 17.99 |
| 43 | Standard | ER culture | 0.4 ng μL−1 | ER F | ER R | 20.79 |
| 44 | Standard | ER culture | 0.01 ng μL−1 | ER F | ER R | 26.87 |
| 45 | Standard | FP culture | 20 ng μL−1 | FP F | FP R | 13.03 |
| 46 | Standard | FP culture | 2 ng μL−1 | FP F | FP R | 16.65 |
| 47 | Standard | FP culture | 0.4 ng μL−1 | FP F | FP R | 21.26 |
| 48 | Standard | FP culture | 0.01 ng μL−1 | FP F | FP R | 26.58 |
| 49 | Standard | MF culture | 20 ng μL−1 | MF F | MF R | 16.25 |
| 50 | Standard | MF culture | 2 ng μL−1 | MF F | MF R | 19.96 |
| 51 | Standard | MF culture | 0.4 ng μL−1 | MF F | MF R | 22.32 |
| 52 | Standard | MF culture | 0.01 ng μL−1 | MF F | MF R | 29.22 |
| 53 | Standard | RI culture | 20 ng μL−1 | RI F | RI R | 14.43 |
| 54 | Standard | RI culture | 2 ng μL−1 | RI F | RI R | 18.51 |
| 55 | Standard | RI culture | 0.4 ng μL−1 | RI F | RI R | 21.58 |
| 56 | Standard | RI culture | 0.01 ng μL−1 | RI F | RI R | 28.97 |
| 57 | Sample | Fecal 1 | NA | AM F | AM R | 17.93 |
| 58 | Sample | Fecal 1 | NA | BC F | BC R | 19.62 |
| 59 | Sample | Fecal 1 | NA | BI F | BI R | 20.73 |
| 60 | Sample | Fecal 1 | NA | BO F | BO R | 16.81 |
| 61 | Sample | Fecal 1 | NA | BT F | BT R | 18.15 |
| 62 | Sample | Fecal 1 | NA | BU F | BU R | 17.56 |
| 63 | Sample | Fecal 1 | NA | CA F | CA R | 23.52 |
| 64 | Sample | Fecal 1 | NA | CS F | CS R | 20.26 |
| 65 | Sample | Fecal 1 | NA | DP F | DP R | 24.39 |
| 66 | Sample | Fecal 1 | NA | EC F | EC R | 20.40 |
| 67 | Sample | Fecal 1 | NA | ER F | ER R | 20.05 |
| 68 | Sample | Fecal 1 | NA | FP F | FP R | 31.88 |
| 69 | Sample | Fecal 1 | NA | MF F | MF R | 20.24 |
| 70 | Sample | Fecal 1 | NA | RI F | RI R | 22.31 |