Table 5.

Components of buffer A

Reagent	For 100 mL of buffer A	Final concentration
NaCl	1.169 g	0.2 M
Trizma Base	2.423 g	0.2 M
EDTA 0.5 M, pH 8.0	4 mL	0.02 M
ddH2O	Up to 100 mL	-