Table 9.

List of bacterial strain-specific primer sequences and corresponding melting temperature of resulting amplicons

Strain/primer pair	Forward primer		Reverse primer		TM
	Primer name	Sequence (5’-->3′)	Primer name	Sequence (5’-->3′)
AM	AM F	GACCGGCATGTTCAAGCAGACT	AM R	AAGCCGCATTGGGATTATTTGTT	85.5°C
BC	BC F	GGCGCATGACATTGGAGGTTT	BC R	AATACGCCGCATCGCTTTTTC	81.6°C
BI	BI F	ACCGGATTCCTATATTGGGCAGTC	BI R	TTCGCTTTTGGCTCTTCCTATTTTC	84.3°C
BO	BO F	GTGAAGGTGCCATCGGAGGAC	BO R	GGACGCTTTGGCCACTATTTCA	83.4°C
BT	BT F	TACTCGCCTCTTTGCAACCCTACC	BT R	GGCCCCAGATCCGAACAACAC	82.8°C
BU	BU F	GCTACCGGGAGATACTGGATTGG	BU R	TGCGGCGGCCTTTGAAC	84.3°C
CA	CA F	GTTCGCGTTCGTTATGGTTGGT	CA R	GTTGAGCTGGGCCGATTGTG	89.4°C
CS	CS F	CCGCTTGGCATGAAACAGGTATC	CS R	TTGGAAGCGGCGAAGAATGG	80.1°C
DP	DP F	TGGCTTCAGGCAAATCTCAAAT	DP R	TCCGGGGAATCAAAACCATAC	83.1°C
EC	EC F	GGTGGCTGGGTGATGTAAAACTGA	EC R	ACCGCCGAGCAAAATGAAGC	87.0°C
ER	ER F	AGCTTGTGCCGCCCATCTCTAT	ER R	TTGCGGTAAAGCTTTGGTGTGG	83.1°C
FP	FP F	TGCCCCCGGGTGGTTCT	FP R	CGTTATTCAAAGCCCCGTTATCAA	80.1°C
MF	MF F	CAGGGATTTTACGTGCTTTATTTTAGTTAT	MF R	AGTTCGGATTCGCTCGTATTTTCT	78.6°C
RI	RI F	TCGAAATTAAAGAGACGGAAACAGAAG	RI R	CCGCTCATATCAATCGAAAACACA	81.9°C