Fig. 2.

Subcellular distribution of untagged, full‐length WT SARS‐CoV‐2 spike and C‐tail mutants in HeLa cells. (A) HeLa cells were transfected with the indicated constructs. After 24 h, cells were fixed, permeabilized, and immunostained with anti‐spike (green) and for the Golgi marker protein giantin (red). (B) HeLa cells, treated as in (A), but without permeabilization to detect only surface localized full‐length spike constructs (green).