Figure 3.

O₂‐Cryogel_VAX recruits high number of CD19+ leukocytes and stimulates B cell expansion in the LNs. A) Photographs of cryogels and LNs at day 56 following subcutaneous injection. The plain circles depict the locations of Cryogel_VAX (blue) and O₂‐Cryogel_VAX (red) under the skin. Immune cell populations in B) cryogels and C) LNs as analyzed by flow cytometry. Two draining LNs and cryogels were analyzed per animal. Values, indicative of individual LNs or cryogels, are presented as mean ± SEM (n = 10). Data were analyzed using two‐way ANOVA and Bonferroni post‐tests to evaluate differences between conditions at the same time point (colored stars indicate statistical significance within a given condition of the same color), *p < 0.05, **p < 0.01, and ***p < 0.001.