Figure 4.

Immunization with O₂‐Cryogel_VAX triggers a balanced Th1/Th2‐mediated immune response against SARS‐CoV‐2. A) Endpoint titers and B) endpoint titer ratios of the different IgG subclasses after 56 days were assessed by ELISA. Th1 and Th2 cytokine levels were measured in mouse serum at C) day 24 and in explanted cryogels at D) day 56 by multiplex assay. E) Flow cytometry gating and frequencies of cytokine‐producing CD44+CD4+ T cells following S and N protein‐derived peptide stimulation of splenocytes isolated at day 21. Data are represented as A–D) mean ± SEM (n = 5) or E) values of individual spleens (n = 5). Data were analyzed using two‐way ANOVA and Bonferroni post‐tests to evaluate differences between groups (colored stars indicate statistical significance within a given condition of the same color), *p < 0.05, **p < 0.01, and ***p < 0.001.