Skip to main content
. 2021 Jul 2;40(15):e107826. doi: 10.15252/embj.2021107826

Figure EV5. Inhibition of IFN and NF‐κB signalling reduces the inflammatory response during SARS‐CoV‐2 infection.

Figure EV5

  • A–C
    Fold gene induction of IL‐6, CXCL10 and IFIT1 24 hpi of Calu‐3 with SARS‐CoV‐2 (MOI 0.04 TCID50VERO/cell) infected in the presence of: (A) 10 μM TPCA‐1 (B) 10 μM PS1145 or (C) 10 μM Ruxolitinib (Rux) with DMSO as control in each case.
  • D–F
    Measurement of SARS‐CoV‐2 (MOI 0.04 TCID50VERO/cell) replication in Calu‐3 in the presence or absence of 10 μM TPCA‐1, 10 μM Ruxolitinib (Rux) or DMSO vehicle as shown measuring (D) genomic and subgenomic E RNA, (E) N +ve cells by flow cytometry, (F) released virus in supernatant (TCID50VERO/cell) at 24 hpi. Mock and SARS‐CoV‐2‐infected/treated conditions were compared by two‐tailed t‐test. *P < 0.05; **P < 0.01; n.s., non‐significant. Mean ± SEM, n = 3.