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. 2021 Jul 2;40(15):e107826. doi: 10.15252/embj.2021107826
Target Sequence
ACE2

Fwd 5′‐CGAAGCCGAAGACCTGTTCTA‐3′

Rev 5′‐GGGCAAGTGTGGACTGTTC‐3′

CCL5

Fwd: 5′‐CCCAGCAGTCGTCTTTGTCA‐3′

Rev 5′‐TCCCGAACCCATTTCTTCTCT‐3′

CXCL10

Fwd 5′‐TGGCATTCAAGGAGTACCTC‐3′

Rev 5′‐TTGTAGCAATGATCTCAACACG‐3′

GAPDH

Fwd 5′‐GGGAAACTGTGGCGTGAT‐3′

Rev 5′‐GGAGGAGTGGGTGTCGCTGTT‐3′

IFIT1/ISG56

Fwd 5′‐CCTCCTTGGGTTCGTCTACA‐3′

Rev 5′‐GGCTGATATCTGGGTGCCTA‐3′

IFIT2

Fwd 5′‐CAGCTGAGAATTGCACTGCAA‐3′

Rev 5′‐CGTAGGCTGCTCTCCAAGGA‐3′

IFNB1

Fwd 5′‐AGGACAGGATGAACTTTGAC‐3′

Rev 5′‐TGATAGACATTAGCCAGGAG‐3′

IFNL1

Fwd 5′‐CACATTGGCAGGTTCAAATCTCT‐3′

Rev 5′‐CCAGCGGACTCCTTTTTGG‐3′

IFNL3

Fwd 5′‐TAAGAGGGCCAAAGATGCCTT‐3′

Rev 5′‐CTGGTCCAAGACATCCCCC‐3′

IL‐1B

Fwd: 5′‐CCTCCTTGGGTTCGTCTACA‐3′

Rev 5′‐GGCTGATATCTGGGTGCCTA‐3′

IL‐6

Fwd 5′‐AAATTCGGTACATCCTCGACG‐3′

Rev 5′‐GGAAGGTTCAGGTTGTTTTCT‐3′

MX1

Fwd 5′‐ATCCTGGGATTTTGGGGCTT‐3′

Rev 5′‐CCGCTTGTCGCTGGTGTCG‐3′

TMPRSS2

Fwd 5′‐CAAGTGCTCCAACTCTGGGAT‐3′

Rev 5′‐AACACACCGATTCTCGTCCTC‐3′

TMPRSS4

Fwd 5′‐ATGCGGAACTCAAGTGGGC‐3′

Rev 5′‐CTGTTTGTCGTACTGGATGCT‐3′

TNF

Fwd 5′‐AGCCTCTTCTCCTTCCTGATCGTG‐3′

Rev 5′‐GGCTGATTAGAGAGAGGTCCCTGG‐3′

SARS‐CoV‐2

E_Sarbeco_F

5′‐ACAGGTACGTTAATAGTTAATAGCGT‐3′

SARS‐CoV‐2

E_Sarbeco_Probe1

5′‐FAM‐ACACTAGCCATCCTTACTGCGCTTCG‐TAMRA‐3′

SARS‐CoV‐2

E_Sarbeco_R

5′‐ATATTGCAGCAGTACGCACACA‐3′