Figure 4.

eIF3e silencing increases GBM cell radiosensitization. (A) GO enrichment analysis of the mRNAs that are upregulated after eIF3e silencing and plotted with REVIGO. Size of the rectangles reflects the P-value (log₁₀). (B, C) Plating efficiency assays measuring the surviving fraction in LN18 (B) or U251 (C) cells treated with control (siScr) or eIF3e (si3e) siRNAs and submitted to a radiation dose scale. Data are presented as mean values ± SEM of three independent experiments; **P < 0.005, ***P < 0.0005 (paired t-test). DEF for U251 and LN18 cells is measured as follows: for the same biological effect (here surviving fraction of log 0.1), the dose on the curve radiation alone (here siScr) is divided by the dose on the curve radiation + treatment (here si3e)). A DEF >1 means that the treatment is functioning as a radiosensitizer.