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. 2020 Jul 31;2(3):zcaa014. doi: 10.1093/narcan/zcaa014

Figure 4.

Figure 4.

Loss of miR-21 reduces the expression of oncogenic let-7 targets. (A) Expression changes of indicated miRNAs in MIR21-KO A549 cells, as determined by miRNA-seq. (B) Representative northern blot of three analyses for miR-21-5p and let-7a-5p in parental (WT) and MIR21-KO A549 cells. 5S served as a loading and normalization control. (C) Scatter plots showing the expression of miR-21 and let-7a in TCGA LUAD patients (n = 519). Pearson correlation coefficient (R) and P-value are indicated. (D) Pearson correlation coefficients of miR-21 with members of the let-7/miR-98 family in the TCGA LUAD cohort. (E) Box plots indicating the expression of let-7a in normal lung tissue (n = 45) and LUAD (n = 519). Statistical significance was determined by the Mann–Whitney test. (F) Representative western blot of three analyses for IGF2BP1, LIN28B and HMGA2 protein expression in parental and MIR21-KO A549 cells. VCL and ACTB served as loading and normalization controls. Statistical significance was determined by Student’s t-test: *P < 0.05; **P < 0.01. (G) Kaplan–Meier plots of overall survival analyses based on expression (best cutoff) of IGF2BP1, LIN28B and HMGA2 mRNAs in LUAD patient samples. HR, hazard ratio; p, log-rank P-value.