Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 22;3(1):zcab009. doi: 10.1093/narcan/zcab009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021. Published by Oxford University Press on behalf of NAR Cancer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Characterization of the BMI1 interactome in GBM. (A) Venn diagram indicating BMI1 interactors in GBM cell lines (blue) and protein networks identified from these proteins, including PcG protein complex, protein translation initiation and elongation, mRNA splicing and AP-2 adaptor complex. (B) Venn diagram indicating BMI1 interactors not shared with RYBP (blue) and protein networks identified from these proteins. (C) Schematic illustrating CRISPR/dCAS9 plasmids to modulate BMI1 expression and western blot with quantification demonstrating BMI1 protein modulation (n = 3; one-way ANOVA, *P< 0.05). (D) Venn diagram showing BMI1 bound proteins, which are also concordantly modulated (blue) and their networks. (E) Bar plot showing PRC1 complex members concordantly modulated with BMI1. Fold change indicates different MS2 binding intensities obtained by calculating Log2 fold change of average MS2 spectra values of different replicates of LN428^iCRBMI1 and U87MG^aCRBMI1 over their matching empty backbones LN428^iCRempty and U87MG^aCRempty respectively (n = 2; two-way ANOVA, *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001). (F) Venn diagram showing BMI1 bound proteins, which are not concordantly modulated (blue) and their networks.